Fruit flies force their young to drink alcohol for protection

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The fruit fly study adds to the evidence “that using toxins in the environment to medicate offspring may be common across the animal kingdom,” says biologist Todd Schlenke.

When fruit flies sense parasitic wasps in their environment, they lay their eggs in an alcohol-soaked environment, essentially forcing their larvae to consume booze as a drug to combat the deadly wasps.

The discovery by biologists at Emory University was published in the journal Science on February 22.

“The adult flies actually anticipate an infection risk to their children, and then they medicate them by depositing them in alcohol,” says Todd Schlenke, the evolutionary geneticist whose lab did the research. “We found that this medicating behavior was shared by diverse fly species, adding to the evidence that using toxins in the environment to medicate offspring may be common across the animal kingdom.”

Adult fruit flies detect the wasps by sight, and appear to have much better vision than previously realized, he adds. “Our data indicate that the flies can visually distinguish the relatively small morphological differences between male and female wasps, and between different species of wasps.”

The experiments were led by Balint Zacsoh, who recently graduated from Emory with a degree in biology and still works in the Schlenke lab. The team also included Emory graduate student Zachary Lynch and postdoc Nathan Mortimer.

The larvae of the common fruit fly, Drosophila melanogaster, eat the rot, or fungi and bacteria, that grows on overripe, fermenting fruit. They have evolved a certain amount of resistance to the toxic effects of the alcohol levels in their natural habitat, which can range up to 15 percent.

Tiny, endoparasitoid wasps are major killers of fruit flies. The wasps inject their eggs inside the fruit fly larvae, along with venom that aims to suppress their hosts’ cellular immune response. If the flies fail to kill the wasp egg, a wasp larva hatches inside the fruit fly larva and begins to eat its host from the inside out.

Last year, the Schlenke lab published a study showing how fruit fly larvae infected with wasps prefer to eat food high in alcohol. This behavior greatly improves the survival rate of the fruit flies because they have evolved high tolerance of the toxic effects of the alcohol, but the wasps have not.

“The fruit fly larvae raise their blood alcohol levels, so that the wasps living in their blood will suffer,” Schlenke says. “When you think of an immune system, you usually think of blood cells and immune proteins, but behavior can also be a big part of an organism’s immune defense.”

For the latest study, the researchers asked whether the fruit fly parents could sense when their children were at risk for infection, and whether they then sought out alcohol to prophylactically medicate them.

Adult female fruit flies were released in one mesh cage with parasitic wasps and another mesh cage with no wasps. Both cages had two petri dishes containing yeast, the nourishment for lab-raised fruit flies and their larvae. The yeast in one of the petri dishes was mixed with 6 percent alcohol, while the yeast in the other dish was alcohol free. After 24 hours, the petri dishes were removed and the researchers counted the eggs that the fruit flies had laid.

The results were dramatic. In the mesh cage with parasitic wasps, 90 percent of the eggs laid were in the dish containing alcohol. In the cage with no wasps, only 40 percent of the eggs were in the alcohol dish.

“The fruit flies clearly change their reproductive behavior when the wasps are present,” Schlenke says. “The alcohol is slightly toxic to the fruit flies as well, but the wasps are a bigger danger than the alcohol.”

The fly strains used in the experiments have been bred in the lab for decades. “The flies that we work with have not seen wasps in their lives before, and neither have their ancestors going back hundreds of generations,” Schlenke says. “And yet, the flies still recognize these wasps as a danger when they are put in a cage with them.”

Further experiments showed that the flies are extremely discerning about differences in the wasps. They preferred to lay their eggs in alcohol when female wasps were present, but not if only male wasps were in the cage.

Theorizing that the flies were reacting to pheromones, the researchers conducted experiments using two groups of mutated fruit flies. One group lacked the ability to smell, and another group lacked sight. The flies unable to smell, however, still preferred to lay their eggs in alcohol when female wasps were present. The blind flies did not make the distinction, choosing the non-alcohol food for their offspring, even in the presence of female wasps.

“This result was a surprise to me,” Schlenke says. “I thought the flies were probably using olfaction to sense the female wasps. The small, compound eyes of flies are believed to be more geared to detecting motion than high-resolution images.”

The only obvious visual differences between the female and male wasps, he adds, is that the males have longer antennae, slightly smaller bodies, and lack an ovipositor.

Further experimentation showed that the fruit flies can distinguish different species of wasps, and will only choose the alcohol food in response to wasp species that infect larvae, not fly pupae. “Fly larvae usually leave the food before they pupate,” Schlenke explains, “so there is likely little benefit to laying eggs at alcoholic sites when pupal parasites are present.”

The researchers also connected the exposure to female parasitic wasps to changes in a fruit fly neuropeptide.

Stress, and the resulting reduced level of neuropeptide F, or NPF, has previously been associated with alcohol-seeking behavior in fruit flies. Similarly, levels of a homologous neuropeptide in humans, NPY, is associated with alcoholism.

We found that when a fruit fly is exposed to female parasitic wasps, this exposure reduces the level of NPF in the fly brain, causing the fly to seek out alcoholic sites for oviposition,” Schlenke says. “Furthermore, the alcohol-seeking behavior appears to remain for the duration of the fly’s life, even when the parasitic wasps are no longer present, an example of long-term memory.”

Finally, Drosophila melanogaster is not unique in using this offspring medication behavior. “We tested a number of fly species,” Schlenke says, “and found that each fly species that uses rotting fruit for food mounts this immune behavior against parasitic wasps. Medication may be far more common in nature than we previously thought.”

http://www.sciencedaily.com/releases/2013/02/130222102958.htm

Baby Wasps Disinfect Cockroaches Before Eating Them

wasp-and-roach

If cockroaches had nightmares, the emerald cockroach wasp surely would deserve a prominent place therein.

These colorful, tiny parasitic wasps sting American cockroaches twice, once in the midsection to prevent them from running away, and a second time directly in the brain, to make the insects sluggish and zombielike. The wasps then drag the roaches by their antenna, akin to a human pulling a dog on a leash, into a protected nook and lay an egg on the roach. The egg ultimately hatches into larvae that devour the roach from the inside out.

About six weeks later, a young adult wasp emerges after spinning a cocoon inside the shell of the roach. But there’s a catch: What’s to prevent the cockroach “meat” from spoiling? Cockroaches are notoriously dirty animals, covered in bacteria that begin to spoil their flesh — and threaten to harm larval wasps — during this long incubation period.

A study published January 7 in the journal the Proceedings of the National Academy of Sciences found that these larval wasps secrete a surprising amount of potent antimicrobial compounds to prevent their cockroach bounty from spoiling.

“They virtually soak their cockroach host with the secretion to inhibit the growth of competitive microbes that would degrade their food and of pathogenic microbes that threaten their lives,” said study co-author Gudrun Herzner, a researcher at Germany’s University of Regensburg.

The study found that Ampulex compressa larvae secrete several types of antibiotics, specifically the chemicals mellein and micromolide, which inhibit the growth of bacteria, fungi and viruses, Herzner told LiveScience.

“On the one hand, the finding is surprising, because such a simple, little insect larva uses such a sophisticated strategy to ward off detrimental bacteria,” Herzner said. “The larvae are like little chemical plants that produce large amounts of different antimicrobial substances.”

However, she continued, it was not really a surprise to find that these parasitic wasps would have evolved to secrete some antimicrobial substances, given that the cockroach is the young wasp’s only food source, which would by itself spoil if not somehow preserved. The wasps live throughout the tropical regions of Africa, Asia and the Pacific.

Micromolide is considered a promising compound to treat Mycobacterium tuberculosis, the microbe that causes tuberculosis, Herzner said.

This is not the only example of insects producing antimicrobial compounds. The European beewolf wasp hunts honeybees, and coats their bodies in an oily substance that inhibits microbes from growing. Certain types of burying beetles also disinfect the carrion they use as larval food. But in both of these cases, the adult animal secretes the antimicrobial chemicals; the emerald cockroach wasp is a rare example of a larval insect making antibiotics, Herzner said.

http://www.livescience.com/26035-wasps-disinfect-cockroaches.html